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                                                                                                                                              首頁 > 新聞中心 > 關于體外消化系統的原理說明
                                                                                                                                              關于體外消化系統的原理說明
                                                                                                                                              點擊次數:966 發布時間:2020-11-19
                                                                                                                                               
                                                                                                                                                體外消化系統可應用到營養學、毒理學、生理學及微生物學等各領域以及食品、藥品、保健品等各個行業。國內外對利用體外消化模型進行相關研究的認可度也越來越高。體外消化系統已被廣泛應用于食品消化過程中物理化學變化,營養物質的生物利用率,腸道運輸和代謝,脂質體等功能活性物質釋放與代謝,益生菌和益生元代謝分析,藥物緩釋釋放研究,重金屬污染物、真菌毒素等有毒物質的代謝研究,動物營養及代謝特性分析等多個領域。
                                                                                                                                                體外消化過程中,人工消化液主要由人工唾液(SimulatedSalivaryFluidSSF)、人工胃液(SimulatedGastricFluidSGF)和人工腸液(SimulatedIntestinalFluidSIF)等消化液組成。人工唾液(SSF)由淀粉酶,電解質溶液組成,人工胃液(SGF)由蛋白酶,模擬胃酸液,磷脂液、電解質溶液等組成。人工腸液(SIF)由胰蛋白酶、胰凝乳蛋白酶、胰脂肪酶、膽汁鹽、胰淀粉酶、電解質溶液組成。
                                                                                                                                                體外消化系統模型和細胞吸收實驗有機耦合得到的結果與人體更加接近,數據更加科學可靠。近年來,細胞模型是研究食物重金屬生物可及性提供新的研究方法。Caco-2細胞源自人結腸癌細胞,具有類似小腸吸收細胞的特征。Caco-2細胞早期研究應用于藥物在小腸吸收的評價和轉運機制的研究中,后研究發現Caco-2單細胞層的轉運吸收量與人體口服吸收實驗的結果表現良好的相關性,先被用于研究金屬離子在小腸上的吸收和轉運機制。利用Caco-2細胞模型耦合體外消化模型研究食物中重金屬在人體的生物可及性,可得到真實客觀的研究結果。
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