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                                                                                                                                              首頁 > 新聞中心 > 如何利用體外模擬消化設備來評定飼料的營養價
                                                                                                                                              如何利用體外模擬消化設備來評定飼料的營養價
                                                                                                                                              點擊次數:1031 發布時間:2019-06-18
                                                                                                                                               
                                                                                                                                                體外消化模擬技術是通過模擬動物消化生理特點,體外模擬消化設備采用與動物體內相近的消化環境和消化酶系,在體外評定飼料消化吸收的一種方法。其優點是:試驗條件可控,干擾因素少,結果的重現性較好,試驗時間短,成本低。但體外消化模擬技術是處于非生理狀態下的儀器檢測,其結果的準確性需要通過體內試驗加以驗證。
                                                                                                                                                雖然飼料養分在禽類體內的消化是一個非常復雜的過程,但與其他家畜(特別是反芻動物)的消化過程相比,還是相對簡單的。酶的消化作用是家禽主要的消化方法,微生物的消化作用較小。因此,用體外消化法評定家禽飼料的營養價值,較其他畜禽相比更具有說服力,營養學家對家禽體外消化方法的研究也取得了許多成功經驗。
                                                                                                                                                人們早是以豬胃蛋白酶和胰酶為消化酶源,在與雞消化道內pH接近的條件下,通過研究不同的胃蛋白酶和胰酶水平以及消化時間對食糜粘性的影響,確立適合于雞的酶法評定飼料營養物質消化率的操作程序。在此基礎上,Zyla等以火雞為試驗動物對該方法進行了改進,實際測定了火雞嗉囊、胃和小腸內食糜的pH作為酶法模擬中的pH參數,不僅對胃和小腸的消化過程進行了模擬,還模擬了雞的嗉囊。同時,還將透析法應用于該方法中,消除消化產物對酶促反應的抑制作用,該方法是目前家禽體外消化模擬方法中較為成熟的一種。荷蘭學者Larsson等(1997)對Zyla等的操作方法進行改進,使用胃腸道動態模型,包括胃、十二指腸、空腸、回腸4個連續模擬室,并通過計算機來調控人工小腸液的分泌及消化環境的調節,使其結果更接近雞消化代謝的試驗結果。人工消化液法由于所有消化酶均來自于人工合成,因此其消化環境適應能力、適反應條件可能都與家禽消化道有所差異,因此,其測定結果與實際家禽飼養試驗結果可能存在系統差異,需要進行校正。
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