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                                                                                                                                              首頁 > 技術與支持 > 介紹體外仿生胃腸道模型的靜態模型
                                                                                                                                              介紹體外仿生胃腸道模型的靜態模型
                                                                                                                                              點擊次數:131 發布時間:2022-09-09
                                                                                                                                                體外模擬胃腸道模型,是對人體或動物的消化道及其消化環境、消化道內的流體動態行為等進行模擬的裝置。與體內試驗相比,體外仿生胃腸道模型作為一種高效的食品(醫藥)配方(處方)和加工工藝篩選工具,不僅能部分甚至*替代體內活體試驗,減少動物犧牲的數量,避免倫理限制,而且可以縮短試驗周期、節約成本、提高結果重復性。此外,體外仿生胃腸道模型可以在時間和空間的任意尺度上,實現對食物與藥物的混合、崩解、釋放、排空、轉運、吸收等動態過程的監測。這對于深刻理解食品與藥物在體內的加工過程極為重要。
                                                                                                                                                靜態模型是目前使用廣泛的體外仿生胃腸道模型,其結構簡單、成本低廉、操作簡便,主要用于研究單一食品組分(如分離的淀粉、蛋白質等)或簡單食品在模擬消化環境中的消化率和營養活性成分的釋放行為。
                                                                                                                                                最常見的靜態模型是由若干個試管、燒杯或錐形瓶等容器組成,用以模擬胃、小腸及大腸。在大部分的靜態模型中,研究人員只是簡單地將均勻分散的食物和模擬胃液充分混合后,置于37℃水浴中攪拌或振蕩1~3小時以模擬胃的消化過程,然后用堿性緩沖液將其pH調至7左右,再與模擬腸液混合2~3小時以模擬小腸的消化過程。
                                                                                                                                                不同的靜態模型對于消化參數,如消化酶濃度、食物與消化酶的比例、pH等的選擇各異,因而不利于不同試驗的結果對比。因此,國際食品消化聯盟于2014年提出了標準化的靜態模型,自提出以來在世界范圍內廣泛引用。該模型以胃腸道相關生理學參數(離子強度、pH、酶濃度、消化時間等)為依據,提出了制備模擬唾液、胃液和小腸液的標準方法,并針對特定食物樣品提供了詳細的操作規程和提示。
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