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                                                                                                                                              首頁 > 技術與支持 > 探討NSP酶對不同類型飼糧的作用效果
                                                                                                                                              探討NSP酶對不同類型飼糧的作用效果
                                                                                                                                              點擊次數:296 發布時間:2022-03-29
                                                                                                                                                非淀粉多糖(NSP)作為一種抗營養因子,常影響畜禽飼糧營養物質的消化。NSP酶由于可降低NSP的抗營養作用,越來越受到關注。許多研究表明,NSP酶能夠降低NSP的抗營養作用,提高營養物質利用率,改善腸道健康。但是也有研究發現,NSP酶對豬生產性能、營養物質利用率沒有影響。由于不同飼糧中NSP的含量和組成差異較大,而NSP酶作為生物反應的催化劑,對底物具有專一性,因此,NSP酶的合理配伍是充分發揮其對飼糧中NSP降解作用的關鍵。但是針對不同的飼糧,通過動物試驗方法來篩選與之相適應的NSP酶的配伍,不但工作量巨大,而且結果變異比較大,不能滿足實際需求。
                                                                                                                                                近年來,一些研究者通過體外模擬消化的方法來研究飼糧中添加NSP酶的效果,為高效、快速的優化NSP酶的配伍提供了一個可行的途徑。胃蛋白酶-胰液素的體外2步模擬消化法對NSP酶在家禽飼糧中的作用效果進行了體外評定,取得了良好的促生產效果。Narasimha等使用體外消化法成功篩選出了纖維素酶、木聚糖酶和β-葡聚糖酶在幾種飼料原料中的最佳組合。但是,使用體外模擬消化法優化生長豬不同飼糧NSP酶譜的研究目前還沒有報道。此外,胰液素是含有多種消化酶的復合物,不同批次之間組成有所不同,無法實現模擬腸液的重復以及測試重演性的需求。本實驗室前期在研究生長豬小腸液組成的基礎上開發了豬模擬小腸液,并在豬飼糧的體外模擬消化上取得了較好的效果。因此,本試驗使用基于生長豬生理消化液組成依據的體外三角瓶2步消化法,在玉米-豆粕型和玉米-雜粕型2種不同類型的生長豬飼糧中使用6種NSP酶,探討NSP酶對不同類型飼糧的作用效果,探索優化豬飼糧中NSP酶配伍的方法,為NSP酶在豬飼糧中的高效使用提供依據。
                                                                                                                                                體外模擬豬胃體外消化法操作過程:
                                                                                                                                                準確稱取1g(過60目篩)飼料樣品(精確至0.0001g),置于50mL三角瓶中,向三角瓶中加入16mL模擬豬胃液,小心混合均勻,用封口膜密封。
                                                                                                                                                放于39℃恒溫水浴搖床中(180r/min)。準確消化4h,完成胃期的模擬消化。
                                                                                                                                                完成胃期模擬消化后,打開封口膜,向三角瓶中準確加入4mL小腸緩沖液,水浴搖床運行5min后,加入預先配制的2mL模擬豬小腸液。封口膜密封,39℃恒溫水浴搖床(180r/min)中進行22h的小腸期模擬消化。取出三角瓶,室溫靜置30min,用事先已絕干稱重的濾紙(Whatman1541,孔徑22~25μm)對三角瓶中的消化殘渣真空抽濾。將抽濾后的殘渣和濾紙置于105℃烘箱中烘干至恒重。
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